dh5α (New England Biolabs)
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dh5α
Dh5α, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3016 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neb+5+alpha+e+coli/NEB+5-alpha+Competent+E%2E+coli/pmc12796543-239-6-7
Average 99 stars, based on 3016 article reviews
Dh5α, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3016 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neb+5+alpha+e+coli/NEB+5-alpha+Competent+E%2E+coli/pmc12796543-239-6-7
Average 99 stars, based on 3016 article reviews
dh5α - by Bioz Stars,
2026-09
99/100 stars
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Polymerase Chain Reaction:Article Title: Profiling of DNA-methylation signatures in human ILCs during homeostasis and allergic disease Article Snippet: Differentially methylated regions (DMRs) and the corresponding promoter region from the HPGDS locus and CRTH2 promoter region containing the enhancer element, as described by , were cloned into the pNL1.1-NanoLuc vector (Promega). .. To this end, PCR-amplified fragments from human PBMC-derived genomic DNA were digested alongside the luciferase vector with compatible restriction enzymes, gel-purified, ligated using T4 DNA ligase (NEB), and transformed into Article Title: SPNS2 exports sphingosine-1-phosphate and imports glucose Article Snippet: Briefly, to prepare plasmid DNA templates the coding region of SPNS2 from Human Tagged ORF Clone in pCMV6-AC-GFP (#RG225940, Origene) was inserted into the expression vector pEU-E01-MCS. .. Plasmid DNA template was isolated from transformed Article Title: SPNS2 exports sphingosine-1-phosphate and imports glucose. Article Snippet: Briefly, to prepare plasmid DNA templates the coding region of SPNS2 from Human Tagged ORF Clone in pCMV6-AC-GFP (#RG225940, Origene) was inserted into the expression vector pEU-E01-MCS. .. Plasmid DNA template was isolated from transformed Luciferase:Article Title: Profiling of DNA-methylation signatures in human ILCs during homeostasis and allergic disease Article Snippet: Differentially methylated regions (DMRs) and the corresponding promoter region from the HPGDS locus and CRTH2 promoter region containing the enhancer element, as described by , were cloned into the pNL1.1-NanoLuc vector (Promega). .. To this end, PCR-amplified fragments from human PBMC-derived genomic DNA were digested alongside the luciferase vector with compatible restriction enzymes, gel-purified, ligated using T4 DNA ligase (NEB), and transformed into Plasmid Preparation:Article Title: Profiling of DNA-methylation signatures in human ILCs during homeostasis and allergic disease Article Snippet: Differentially methylated regions (DMRs) and the corresponding promoter region from the HPGDS locus and CRTH2 promoter region containing the enhancer element, as described by , were cloned into the pNL1.1-NanoLuc vector (Promega). .. To this end, PCR-amplified fragments from human PBMC-derived genomic DNA were digested alongside the luciferase vector with compatible restriction enzymes, gel-purified, ligated using T4 DNA ligase (NEB), and transformed into Article Title: Prototyping Minimal Extracellular Vesicle Mimetics Using Cell-Free Synthesis. Article Snippet: The generated plasmids were transformed into NEB 5-alpha Competent E. coli (C2987H, New England Biolabs). .. Transformation was performed by incubating 10 ng of the plasmid with 10 μL of competent Article Title: Modified meiosis in the tardigrade Hypsibius exemplaris maintains heterozygosity across the genome Article Snippet: Final products from 2-step PCR performed on pooled tardigrade samples (15-20 animals each) were purified using the Zymoclean Gel DNA Recovery Kit (Zymo D4008) and quantified using Nanodrop. .. Each amplicon was cloned into a pDEST17 Gateway destination vector (Invitrogen 11803012) using the NEBuilder HiFi DNA Assembly Master Mix kit (NEB E2621), and plasmids were transformed into chemically-competent Article Title: SPNS2 exports sphingosine-1-phosphate and imports glucose Article Snippet: Briefly, to prepare plasmid DNA templates the coding region of SPNS2 from Human Tagged ORF Clone in pCMV6-AC-GFP (#RG225940, Origene) was inserted into the expression vector pEU-E01-MCS. .. Plasmid DNA template was isolated from transformed Article Title: SPNS2 exports sphingosine-1-phosphate and imports glucose. Article Snippet: Briefly, to prepare plasmid DNA templates the coding region of SPNS2 from Human Tagged ORF Clone in pCMV6-AC-GFP (#RG225940, Origene) was inserted into the expression vector pEU-E01-MCS. .. Plasmid DNA template was isolated from transformed Transformation Assay:Article Title: Profiling of DNA-methylation signatures in human ILCs during homeostasis and allergic disease Article Snippet: Differentially methylated regions (DMRs) and the corresponding promoter region from the HPGDS locus and CRTH2 promoter region containing the enhancer element, as described by , were cloned into the pNL1.1-NanoLuc vector (Promega). .. To this end, PCR-amplified fragments from human PBMC-derived genomic DNA were digested alongside the luciferase vector with compatible restriction enzymes, gel-purified, ligated using T4 DNA ligase (NEB), and transformed into Article Title: Prototyping Minimal Extracellular Vesicle Mimetics Using Cell-Free Synthesis. Article Snippet: The generated plasmids were transformed into NEB 5-alpha Competent E. coli (C2987H, New England Biolabs). .. Transformation was performed by incubating 10 ng of the plasmid with 10 μL of competent Article Title: Modified meiosis in the tardigrade Hypsibius exemplaris maintains heterozygosity across the genome Article Snippet: Final products from 2-step PCR performed on pooled tardigrade samples (15-20 animals each) were purified using the Zymoclean Gel DNA Recovery Kit (Zymo D4008) and quantified using Nanodrop. .. Each amplicon was cloned into a pDEST17 Gateway destination vector (Invitrogen 11803012) using the NEBuilder HiFi DNA Assembly Master Mix kit (NEB E2621), and plasmids were transformed into chemically-competent Article Title: SPNS2 exports sphingosine-1-phosphate and imports glucose Article Snippet: Briefly, to prepare plasmid DNA templates the coding region of SPNS2 from Human Tagged ORF Clone in pCMV6-AC-GFP (#RG225940, Origene) was inserted into the expression vector pEU-E01-MCS. .. Plasmid DNA template was isolated from transformed Article Title: SPNS2 exports sphingosine-1-phosphate and imports glucose. Article Snippet: Briefly, to prepare plasmid DNA templates the coding region of SPNS2 from Human Tagged ORF Clone in pCMV6-AC-GFP (#RG225940, Origene) was inserted into the expression vector pEU-E01-MCS. .. Plasmid DNA template was isolated from transformed Article Title: Investigating and correcting a rare pathogenic mutation in GDF11. Article Snippet: Transformations were performed using NEB 5-alpha Competent E. coli (NEB #C2987I) according to manufacturer’s instructions. ngRNA Plasmid Digest – BPK1520 was digested with BsmBI-v2 (NEB #R0739S) at 55°C for 1 h, run on a 1% agarose gel, and the ~2.2 kb fragment was purified (Promega Wizard SV Gel and PCR Clean-Up System, #A9282). pegRNA Plasmid Digest – pU6-pegRNA-GG-acceptor, pU6-tevopreq1-GG-acceptor, and pU6-tmpknot-GG-acceptor were digested with BsaI-HFv2 (NEB #R3733S) at 37°C for 1 h, run on a 1% agarose gel, and the ~2.2 kb fragment was purified. .. Oligo Duplex Annealing – ssDNA oligos for pegRNAs and ngRNAs (IDT; sequences in Supplemental Information) were annealed as described in [5]. ngRNA Cloning – Annealed duplex oligos were ligated into BsmBI-v2-digested BPK1520 using T4 DNA ligase (NEB #M0202), transformed into Amplification:Article Title: Modified meiosis in the tardigrade Hypsibius exemplaris maintains heterozygosity across the genome Article Snippet: Final products from 2-step PCR performed on pooled tardigrade samples (15-20 animals each) were purified using the Zymoclean Gel DNA Recovery Kit (Zymo D4008) and quantified using Nanodrop. .. Each amplicon was cloned into a pDEST17 Gateway destination vector (Invitrogen 11803012) using the NEBuilder HiFi DNA Assembly Master Mix kit (NEB E2621), and plasmids were transformed into chemically-competent Clone Assay:Article Title: Modified meiosis in the tardigrade Hypsibius exemplaris maintains heterozygosity across the genome Article Snippet: Final products from 2-step PCR performed on pooled tardigrade samples (15-20 animals each) were purified using the Zymoclean Gel DNA Recovery Kit (Zymo D4008) and quantified using Nanodrop. .. Each amplicon was cloned into a pDEST17 Gateway destination vector (Invitrogen 11803012) using the NEBuilder HiFi DNA Assembly Master Mix kit (NEB E2621), and plasmids were transformed into chemically-competent other:Article Title: Integrase-On-Demand: bioprospecting integrases for targeted genomic insertion of genetic cargo. Article Snippet: Donor (Ec100D pir + cloning trains) and helper (HB101 with conjugal plasmid pRL443) sc heric hia coli strains for cyanobacterial conjugation were ultured at 30 ◦C in LB Lennox medium (Sigma, L3022× 1KG). Cell Culture:Article Title: Integrase-On-Demand : bioprospecting integrases for targeted genomic insertion of genetic cargo Article Snippet: Donor (Ec100D pir + cloning strains) and helper (HB101 with conjugal plasmid pRL443) Escherichia coli strains for cyanobacterial conjugation were cultured at 30°C in LB Lennox medium (Sigma, L3022-6 × 1KG). .. Isolation:Article Title: SPNS2 exports sphingosine-1-phosphate and imports glucose Article Snippet: Briefly, to prepare plasmid DNA templates the coding region of SPNS2 from Human Tagged ORF Clone in pCMV6-AC-GFP (#RG225940, Origene) was inserted into the expression vector pEU-E01-MCS. .. Plasmid DNA template was isolated from transformed Article Title: SPNS2 exports sphingosine-1-phosphate and imports glucose. Article Snippet: Briefly, to prepare plasmid DNA templates the coding region of SPNS2 from Human Tagged ORF Clone in pCMV6-AC-GFP (#RG225940, Origene) was inserted into the expression vector pEU-E01-MCS. .. Plasmid DNA template was isolated from transformed Purification:Article Title: SPNS2 exports sphingosine-1-phosphate and imports glucose Article Snippet: Briefly, to prepare plasmid DNA templates the coding region of SPNS2 from Human Tagged ORF Clone in pCMV6-AC-GFP (#RG225940, Origene) was inserted into the expression vector pEU-E01-MCS. .. Plasmid DNA template was isolated from transformed Article Title: SPNS2 exports sphingosine-1-phosphate and imports glucose. Article Snippet: Briefly, to prepare plasmid DNA templates the coding region of SPNS2 from Human Tagged ORF Clone in pCMV6-AC-GFP (#RG225940, Origene) was inserted into the expression vector pEU-E01-MCS. .. Plasmid DNA template was isolated from transformed Cloning:Article Title: Investigating and correcting a rare pathogenic mutation in GDF11. Article Snippet: Transformations were performed using NEB 5-alpha Competent E. coli (NEB #C2987I) according to manufacturer’s instructions. ngRNA Plasmid Digest – BPK1520 was digested with BsmBI-v2 (NEB #R0739S) at 55°C for 1 h, run on a 1% agarose gel, and the ~2.2 kb fragment was purified (Promega Wizard SV Gel and PCR Clean-Up System, #A9282). pegRNA Plasmid Digest – pU6-pegRNA-GG-acceptor, pU6-tevopreq1-GG-acceptor, and pU6-tmpknot-GG-acceptor were digested with BsaI-HFv2 (NEB #R3733S) at 37°C for 1 h, run on a 1% agarose gel, and the ~2.2 kb fragment was purified. .. Oligo Duplex Annealing – ssDNA oligos for pegRNAs and ngRNAs (IDT; sequences in Supplemental Information) were annealed as described in [5]. ngRNA Cloning – Annealed duplex oligos were ligated into BsmBI-v2-digested BPK1520 using T4 DNA ligase (NEB #M0202), transformed into |
